Análise de lesões inflamatórias intestinais associadas a mycobacteriúm avium subsp. paratuberculosis em caprinos e ovinos no Semiárido do Piauí

Abstract

Johne's disease or paratuberculosis, as it is known, is caused by a bacillus, Mycobacterium avium subsp. Paratuberculosis (MAP), which affects ruminants in general, causing chronic granulomatous enteritis. Therefore, objective was to investigate the occurrence of inflammatory bowel lesions associated with paratuberculosis in sheep and goats in Chapada do Vale do Itaim region, in Piauí state. To carry out this research, small and large intestine fragments (ileum, ileocecal valve, and cecum) were collected, respectively, from 30 animals. Samples were histologically processed and stained with hematoxIlin and eosin to check for possible lesions, in addition to a protocol for staining acid-alcohol resistant bacilli using Ziehl Neelsen’s technique. In the histopathological evaluation, 12 animals were suggestive of lesions associated with paratuberculosis, in which it was possible to identify inflammatory infiltrates with macrophages, lymphocytes, monocytes and neutrophils presence, in addition, granulomas and Langhans giant cells. On smear microscopy, two animals were suggestive of acid-fast bacillus (AFB). Descriptive method was used for statistical analysis. Therefore, it is concluded that animals evaluated were suggestive over paratuberculosis occurrence.