Estudo circadiano dos metabólitos secundários voláteis de Bougainvillea spectabilis (Nyctaginaceae) da cidade de Parnaíba-PI

Abstract

The species Bougainvillea spectabilis is endemic to South America, can be found in most Brazilian states. It’s a thorny shrub with yellowish white flowers, used as part of decoration and recidence protection. In the literature, several works showed biological activity of this plant species as antihyperglycemic, antiviral, insecticidal activities, among others. The present work aimed to investigate the variation of the volatile secondary metabolites of leaves of B. spectabilis through of cicardian study. The essential oils of B. spectabilis leaves were extracted from the same specimen at three different times (08:00, 12:00 and 16:00 hours), using the hydrodetilation technique in a Clevenger dosing device modified by OR Gottlieb, and analyzed by Gas Chromatography coupled to Mass Spectrometry. The yield of the oil obtained increased throughout the day, with an increase of approximately 50% between the highest and the lowest yield. In the essential oils of the hours of 08:00 and 16:00 h, twelve volatile metabolites were detected. Whereas in the hour of 12:00 h only eleven compounds were obtained. Seven simultaneous compounds were identified at extraction times, but with different percentages, such as hexanol and linalol, whose values for the hours of 08:00 h, 12:00 h, and 16:00 h correspond respectively to: 16.06 - 18.10 - 15.62%; 3.29 - 2.93 - 2.00%. In this study, six major compounds were identified: (E) -hex-2-en-1-ol, (Z) -hex-3-en-1-ol acetate, hexanol, methyl (Z) formate salicylate of hex-3-en-1-ol and 7-hydroxyheptene. However, only hexanol was identified as major in all essential oils obtained in the circadian study.