Potencial antimicrobiano de nanopartículas de prata AgNPs estabilizada em extrato aquoso de Bauhinia forficata

Abstract

The increase in the number of microorganisms resistant to antibiotics is considered a global health problem with devastating impacts on society. The expansion of this problem consists of the incorrect use of medicines available by pharmaceutical industries, resulting in difficulties in treating infectious diseases that spread rapidly. The number of deaths from untreatable infections caused by these resistant pathogens highlights the need to develop new treatments, synthetic routes and possibilities to combat antimicrobial resistance. In this sense, the objective of this research is to synthesize silver nanoparticles (AgNPs) prepared by the green synthesis method using aqueous extract of Bauhinia Forficata; characterize them through spectroscopy in the visible ultraviolet region (UV-VIS), x-ray diffraction (XRD), spectroscopy in the infrared region (FTIR), dynamic light scattering (DLS) and zeta potential; in addition to evaluate its potential antimicrobial effect. Characterization by spectroscopy in the ultraviolet visible region (UV-VIS) and x-ray diffraction (XRD) identified the material; its interaction with the extract was analyzed by infrared spectroscopy (FTIR); through dynamic light scattering (DLS) and zeta potential, the hydrodynamic radius was measured and the stability of the silver nanoparticles formed was evaluated; then they were applied against bacterial strains: Staphylococcus aureus and Escherichia coli and fungal species: Candida albicans and toxicological test. The results show a maximum absorption peak of 438 nm, stability after 1 day and 7 days of synthesis and a maximum diffraction peak at 38°. The material formed remained electronically stable with an average zeta potential of 32,1 mV, PDI of 1,5 and average hydrodynamic diameter of 21,2 nm. Furthermore, the diffusion susceptibility test in the well proves significant antimicrobial activity of AgNPs against bacterial strains and fungal infection with the formation of 29 mm inhibition halos in the tested cultures.