Preparo e caracterização de membranas de quitosana com extratos foliares de combretum duarteanum cambess para aplicações biológicas

Abstract

Considering the possibility of improving the performance of chitosan membranes, some compounds with additional pharmacological potential are being incorporated into this biomaterial. Thus, the objective of this work was to prepare and characterize chitosan membranes with aqueous and ethanolic leaf extract (M.Q.ExAquo/ M.Q.ExEtOH) of White-branch (Combretum duarteanum Cambess) for biological applications. The membranes were prepared using powdered chitosan dissolved in acetic acid and characterized by X-ray fluorescence spectroscopy (FRX), infrared spectroscopy (FTIR), scanning electron microscopy (SEM), Thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), wettability, absorption test and antimicrobial test. The FRX showed that the membranes of chitosan with extracts present higher content of sodium, magnesium and calcium. The FTIR spectrum showed bands characteristic of chemical clusters of both chitosan and the incorporated extracts. For the membranes with extracts a SEM revealed uneven and porous surfaces. As analyzes of TGA and DSC show that the membranes of chitosan with extracts present greater thermal stability. The wettability test showed that all surfaces tested were predominantly hydrophilic and that M.Q.ExAquo showed higher surface tension. As for the water and saliva absorption, it was verified that with 48h of immersion a pure chitosan membrane had greater mass gain. It was possible to observe that the membranes incorporated with extracts showed to be efficient inhibitors of the microbial growth. From these analyzes, the effective incorporation of the extracts of C. duarteanum into the chitosan, with the obtaining of membranes with porous surfaces, could be a favorable characteristic in the process of cell adhesion and proliferation, and that bioactive substances of the extracts make the Membranes more resistant to pathogenic microorganisms and decomposition in liquids. This process of incorporating extracts into chitosan is an alternative to improve the performance and use of these healing membranes.