Preparo e caracterização de membranas de quitosana com incorporação do extrato hidroalcoólico liofilizado do chenopodium ambrosioides para aplicação como biomaterial

Abstract

The challenge to obtain healing biomaterials is developing biomaterial with appropriate characteristics to the treatment. In addition, materials from renewable sources with easy and inexpensive production process are desirable. Therefore, the purpose of this work was to produce and characterize chitosan based membranes with the incorporation of lyophilized hydroalcoholic extract of Chenopodium ambrosioides to its use as cicatrizing biomaterial. Chitosan power and acetic acid was used for the preparation of the pure membranes (mem P/C). Incorporated membranes (mem E/C) were produced by adding 0.5% and 1% of hydroalcoholic extract of C.ambrosioides to the same formulation of the pure membranes. It was used Fourier Transform InfraRed (FTIR) spectroscopy to characterize the C. ambrosioides extract. The membranes were characterized by FTIR, Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA), Differential Exploration Calorimetry (DSC), water absorption test and wettability test (sessile gout technique). The cytotoxicity of the membranes was evaluated by the MTT test and cytotoxicity on sheep erythrocytes. Membranes with macroscopic appearance were obtained: continuous, homogeneous and flexible. The FTIR showed that the O–H binding band was widened in the membranes E/C. Regarding mem C/E the SEM revealed morphology more heterogeneous than mem P/C and the presence of pores in membranes with lower concentration of extract (mem E/C 0.5%). TGA and DSC analysis showed similar thermal behavior for both the P/C and mem c/e 0.5% and higher thermal stability for 1% E/C membranes. Membranes with incorporated extract absorbed more water than pure membranes. The wettability test showed that all the samples had a hydrophilic character and that the surface tension and polar component increased with the presence of the extract. The MTT test in murine macrophages and cytotoxicity in sheep erythrocytes shows no cytotoxicity to the membranes. Thus membranes E/C present satisfactory conditions for application as a dermal healing dressing