Estudo circadiano do óleo essencial da espécie Eugenia punicifolia (Kunth) DC. do Município de Ilha Grande do Piauí

Abstract

The species Eugenia punicifolia (Kunth) is an aromatic shrub of the family Myrtaceae, popularly known as murta and field cherry, popularly used as a medicinal plant. With the objective of extract and identify the volatile constituents of this species, a circadian study of the essential oil of the leaves of Eugenia punicifolia (Kunth) of the municipality of Large Island from Piauí was carried out. The essential oils were extracted from the same exemplary at three different times (08:00, 12:00 and 16:00 hours), using the Hydrodetilation technique in a Clevenger dosing device modified by OR Gottlieb, and analyzed by Coupled Gas Chromatography Mass Spectrometry. The yield obtained of the oils had a considerable variation, being the best yield obtained in the collection of 16:00h (0,087%). The extracted oils presented variations in their volatile chemical constituents, being identified 59 different compounds in the circadian study. Among them, eleven compounds presented major contents, that is, equal to or above 3% (α-pinene, (1S)-6,6-dimethyl-2-methylenebicyclo[3.1.1]heptane, (Z)-3,1-methylethyl-2-(1-methylethenyl)-4-cyclohexanecarboxy late, 1-ethenyl-1-methyl-7-dimethyl-1,3,6-triene, 3,7,7-trimethylbicyclo [4.1.0]hept-2ene, 1-ethenyl-1-methyl-2-(1-methylethenyl)-4-(1-methylethylidene) cyclohexane, caryophyllene, [3aS-(3a.alpha.,3.beta.,4.beta.,7.alpha.,7.alpha.-octahydro-7-methyl3-methylethyl)-1H-cyclopenta[1,3]cyclopropa[1,2]benzene, [1S-(1.alpha.,2.beta.,4.be ta.]-1-ethenyl-2,4-bis(1-methylethyl)cyclohexane, caryophyllene oxide, 1-methyl-2-(1methylethyl)benzene and (E)-3,7-dimethyloct-1,3,6-triene), highlighting the caryophyllene with the highest percentage in the three collection times. The study is important because it will serve as the basis for future researchers who want to study pharmacological/biological activities as antifungal, anti-inflammatory and toxicity of the species.