Desenvolvimento de filmes de Quitosana/PVA/Norbixina com potencial aplicação em regeneração de tecidos

Abstract

Chitosan films have been used as scaffolds in regenerative medicine because they are biocompatible and biodegradable and its association with synthetic polymers and substances with acknoledged biological activities has been reported. The objective of this study was to develop scaffolds of chitosan (CF), chitosan/Norbixin (FCN), chitosan/PVA (FCP) and chitosan/PVA/Norbixin (FCPN) plasticized with glycerol using the solvent casting technique. Scaffolds and norbixin extracted from annatto seeds (Bixa orellana L.) were studied by ultraviolet-visible spectrophotometry (UVVis), infrared (IR), X-ray diffraction (XRD), fluorescence (FRX), thermogravimetric (TG), derivative thermogravimetry (DTG) and scanning electron microscopy with coupled energy dispersion spectroscopy (SEM-EDS). Norbixin analyzes showed that the extraction was performed satisfactorily. The analysis of the scaffolds by FTIR showed intermolecular interactions among film components. The TG-DTG results showed that the use of glycerol and norbixin in the formulation of the FQ, FQN and FQPN films cause the reduction of thermal stability. In contrast in the FQP and FQPN films the addition of PVA provided the increase in thermal stability and barrier properties when compared to the FQ film. The XRD results showed changes in the crystallographic profile of the films in relation to pure chitosan. SEM analysis showed that the films revealed a compact surface, without cracking. It also showed that the addition of PVA to the chitosan matrix become the surface of the film rough indicating the separation of phases among the componentes, and it suggests good adhesion and cell proliferation. The incorporation of norbixin into the chitosan film (FQN) makes the surface smoother in relation to the CF film. The EDS results showed that in addition to the constituent elements of the components of the C, N, and O films were detected Na and Si. In order to obtain smoother and more flexible homogeneous films by intermolecular interactions between the components, the films FQN and FQPN obtained better thermal degradation in relation to the films FQ and FQP respectively.